The behind-the-scenes pictures were taken during the band's famous 1965 US tour by musician Alan Holmes, a member of Beatles' support act Sounds Inc.
They capture the Fab Four on stage, at press conferences as well as during the filming of the famous Ed Sullivan Show.
The collection will go under the hammer later this month and is expected to fetch £5,000.
Pictures of screaming fans at the 1965 Shea Stadium concert also feature in the collection
The lot of 38 original prints, 12 rolls of film and colour transparencies, all taken in 1964 and 1965, will be sold with full copyright.
Pictures from the 1965 Shea Stadium concert also feature in the lot, where the noise of some 55,000 fans was said to be so deafening neither the band nor the crowd could hear a note of what was being played on stage.
Mr Holmes never published the images in his lifetime and the collection was passed to a friend following his death.
Source: BBC News/bbc.com